Liquid Dosage
From oral vaccines to immune-modulators and offering a broad range of applications in biotechnology and pharmaceutical research, development and analysis, bacterial lysates are widely used in the life science industries. Different methods can be used to prepare cell lysates from Escherichia coli (E. coli) cells, for example, including sonication, homogenization, enzymatic lysis and freezing/grinding.
Homogenizers, however, are the most commonly used devices to lyse large quantities of bacteria. A cell suspension is pumped through the minute orifice of a homogenizing valve at high pressure; this is followed by the sudden release of the pressure on the suspension, resulting in an instant expansion that provides the turbulence and liquid shear — combined with the high pressure — that breaks the cells.
The main disruptive factor in this process is the pressure applied to the sample and the subsequent pressure drop across the valve. This provides the impact and the shear stress, making the cells break in proportion to the operating pressure.
Compared with other methods, homogenization is both convenient and efficient, offering higher percentage cell disruption rates. Modern GEA homogenizers are able to work continuously, enabling large quantities of cells to be processed at high operational pressures, which eliminates the need to add deoxyribonuclease (DNase) to the cell suspension.
Suggested pressures for typical applications are 600–1000 bar with a single stage homogenizer; multiple passes (2–3) are generally required to achieve adequate lysis. It should be noted that high processing pressures result in a rise in operating temperatures. Cells should, therefore, be cooled (4 °C) prior to use to avoid inactivating proteins by foaming.
Fermenters, separators, decanters and cross-flow filtration technology for the gentle treatment of fragile cell cultures are also available from GEA. We understand that the careful treatment of living micro-organisms, sterility and efficiency are prerequisites to economical, reliable and efficient processing.
Visualizzazione di 4 su 10
Le testate di compressione di GEA sono il cuore pulsante dell'omogeneizzatore poiché attivano il processo di omogeneizzazione pompando il prodotto ad alta pressione per convogliarlo all'interno della valvola di omogeneizzazione.
GEA Hilge offre una gamma versatile di pompe centrifughe e volumetriche per un'ampia varietà di applicazioni sensibili nell'industria delle bevande, alimentare e farmaceutica.
GEA's thorough understanding of the lyophilization process enables them to supply a comprehensive range of products and services, comprising laboratory equipment, pilot plants for research and small-scale production batches, industrial size pharmaceutical production freeze dryers.
Gli omogeneizzatori da laboratorio GEA, piccoli e compatti, consentono di testare gli effetti dell'omogeneizzazione sul prodotto e valutare quali sono i migliori parametri di processo.
Il cambiamento climatico e la crescita della popolazione mondiale mettono sotto pressione l'industria alimentare, che consuma molta energia, per nutrire più persone senza impattare ulteriormente sul pianeta. George Shepherd, Global Technical Sustainability Manager di GEA, spiega come GEA utilizzi il proprio know-how ingegneristico per aiutare le aziende di trasformazione a produrre in modo più sostenibile, aumentando al contempo la produttività.
The world's population is growing and with it demand for milk. Dairy is an essential component of many global diets. However, its production can be resource-intensive and impact the environment. GEA’s Christian Müller, Senior Director Sustainability Farm Technologies, sheds light on how technological innovations powered by GEA make milk production more efficient and profitable.
Ogni bevanda e alimento sicuro è una vittoria contro invisibili minacce microbiche, il risultato di una battaglia condotta sulla scorta di un secolo di progettazione di processi igienici. Con oltre 100 anni di know-how ingegneristico e di progettazione igienica, GEA stabilisce lo standard industriale per attrezzature di lavorazione che proteggono gli alimenti e salvano vite.